Composite

Part:BBa_K199026

Designed by: Kin Lau   Group: iGEM09_MoWestern_Davidson   (2009-07-08)


Tetrev-RBSrev-pLux

This part is inverted RBS-Tet ligated upstream of pLux. This is intended to measure the backward activity of pLux.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 897
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 503
    Illegal NgoMIV site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1220


[edit]
Categories
Parameters
None